CHAIR & Associate Professor of Biology
Spencer Hall 167A/ext. 1747
RESEARCH & SCHOLARSHIP
Research in my laboratory focuses on understanding the underlying toxicity of human polyglutamine-containing proteins that are associated with numerous neurodegenerative diseases. The most well-studied of the polyglutamine diseases is Huntington’s Disease, whose genetic determinant is an expansion of a CAG triplet repeat in the gene encoding the Huntingtin protein.
To study how and why the resultant mutant proteins are toxic to cells, we express the human disease proteins in different tissues of the invertebrate model system, C. elegans. The ease of generating transgenic lines, the availability of mutant strains, and the relatively simple body plan of C. elegans makes it an especially useful tool to address our research questions.
My students and I approach our research questions using the following techniques:
- Fluorescence microscopy
- Classical genetics and RNAi-mediated gene knockdown
- Quantitative real-time PCR (qRT-PCR)
- Fluorescent reporters to monitor gene expression
- Immunoblot analysis of protein accumulation
- Biochemical and biophysical approaches to study protein solubility
- Motility assays to measure toxicity
FELLOWSHIPS, HONORS, & GRANTS
McCrickard Faculty Development Grant
*Christie, N., *Lee, A., Gray, A., Fay, H., Kikis, E.A. (2014) Novel Polyglutamine Model Uncouples Proteotoxicity from Aging. PLoS ONE 9(5): e96835 *authors contributed equally
Gidalevitz, T., Kikis, E.A., Morimoto, R.I. (2010) A cellular perspective on conformational disease: the role of genetic background and proteostasis networks Curr. Opin. Struct. Biol. 20(1): 23-32.
Kikis, EA., Ben-Zvi, A., Morimoto, RI. “C. elegans as a Model System to Study the Biology of Protein Aggregation and Toxicity.” Protein Misfolding Diseases: Current and Emerging Principles and Therapies. Ed. Dobson, CM., Kelly, JW., and Ramirez-Alvarado, M. (2010).